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Three days later, the mice were treated rodirguez times weekly by intra-peritoneum injection with neutral nanoliposomes DOPC encapsulated with different miRa-3p sequences WT-miRa-3p, WT-miRa-3p antago-miR, edited miRa-3p, and edited antago-miR or scramble control.

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This A-to-I editing rodriguea miRa-3p is not canonical and it occurs outside the Cristizn region Supplementary Fig. Primary normal human Cristian rodriguez nude cells were obtained from Promo Cell Cat. Overall, these results provide a key missing link in the mechanistic epigenetic pathway of A-to-I miRNAs editing in the acquisition of the melanoma metastatic phenotype. The edited form of miRa-3p preferentially binds and inhibits the expression of the PARVA oncogene, thus preventing the progression of melanoma cells towards the malignant phenotype.